English translation for "cloned sequence"
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- 克隆片段
克隆序列
Related Translations:
cloning: 基因转殖 克隆克隆繁殖克隆技术克卢克隆化无性繁殖系化无性系化
- Example Sentences:
| 1. | And many important cis - acting regulatory elements were found in the cloned sequence region by plantcare software analysis . ref promoter was predicted to be most probably induced by light , hot , gibberellin , ethenen or wound
经plantcare软件分析发现序列中含有多种调控元件，经预测ref启动子可能被光、热、 ga 、乙烯或伤所诱导。 | | 2. | The activity of the invertase of three high - sugar saccharomyces cerevisiae strains were investigated and their amino acid sequence were determined by the use of modern biotech including enzymatic activity determination , specific pcr , the cloning sequencing and bio - information analysis etc
摘要采用现代生物技术中的酶活测定、特异pcr 、克隆测序和生物信息分析等对比研究3株高糖酵母蔗糖转换酶活性及其氨基酸序列。 | | 3. | Two test sets were constructed by mapping the newly published 28 , 469 full - length kome rice cdna to the rgp bac clone sequences of oryza sativa ssp . japonica : a single - gene set of 550 sequences and a multi - gene set of 62 sequences with 271 genes
我们把日本kome数据库中的28469个全长cdna同国际水稻基因组计划的粳稻bac序列对比，经过严格选择构建了两个测试数据集合： 550条单基因序列组成ossng550集合，包含271个基因的62条多基因序列组成osmtg62集合。 | | 4. | After electrophorised on 1 % agarose gel , the pcr production was purified with agarose gel dna extraction kit . the segment was ligated with vector pmd18 - t and then was tranformed into the competent cell of dh5 a . a construction mstnd - pmd18t was generated by inserting the sequence of 254bp into pmd18 - t vector and selecting the sense clones . positive clone was identified by three ways : endonuclease digestion , pcr and sequencing . the result showed that the cloned sequence coincides with the designed sequence . this construction was digested with nco i and xho i and ligated the pet28a ( + ) vector digested with the same enzymes using dna ligation kit . the production of ligation reaction was transformed into the competent cell of bl21 ( de3 ) . after 12 - 16 hours of culture , several colnes appeared on the plate . some positive clones were selected to extract their plasmid . these plasmids were digested by nco i and xho i and indentified by pcr . a contraction , mstnd - pet28a was generated . the result showed that the cloned sequence coincides with the designed sequence
F _ 1长38bp ， r _ 1长36bp ，其它片段均40bp长， f _ 1和r _ 1片段两端分别加上限制性内切酶nco和xho的识别位点序列。用成对单链片段进行延伸反应，然后用其他单链片段作为引物，进行pcr扩增，用dna快速纯化回收试剂盒回收所得254bppcr产物，与pmd18 - t载体连接、转化dh _ 5 。受体菌感受态细胞，利用蓝白斑遗传学筛选法筛选阳性克隆，提取其质粒，采用nco和xho双酶切鉴定，获得了254bp的片段；用pmd18 - t载体上的特异引物rv - m和m13 - 47进行pcr鉴定，获得300bp的片段。 | | 5. | In order to use the responding ability to the inducers of pr - la promoter , two fragments , ip1 ( 900 bp ) and ips ( 603 bp ) were cloned from tobacco genomic dna by polymerase chain reaction ( pcr ) with primers designed according to the sequence reported in genbank . sequence analysis of the amplified 900 bp fragment indicated that the cloned sequence had 99 % of homology to the known sequence . its transcription start site , tata box and consensus sequence " tgac " conserved in pr genes were identical to those of pr - la promoter
根据pr - 1a基因的报道序列，设计两对引物，以烟草基因组为模板，通过pcr扩增得到900bp ( ipl )和603bp ( ips )两个目标片段，序列测定表明克隆的启动子与报道序列具有99的同源性，转录起始位点、 tatabox及保守序列tgac与报道序列均完全相同。 | | 6. | In our experiment , the specific fragment was amplified from transgenic bobwhite genome dna at annealing temperature 61 by using high - fidelity pfu dna polymerase and cloned into clone vector pgem - 7fz ( + ) , then sequenced . the cloned sequence was completely identical to the sequence which was issued in genbank
本实验采用了高保真pfudna聚合酶，在退火温度61条件下从转基因bobwhite品种基因组dna中扩增出特异性片段，将此片段插入克隆载体pgem - 7fz ( + ) ，经测序和序列分析表明，所扩增得到的片段含有bar基因完整的读码框，并且序列与genbank中发表的序列完全一致。 |
- Similar Words：
- "cloned dna" English translation, "cloned gene" English translation, "cloned hybrid cell" English translation, "cloned line" English translation, "cloned node" English translation, "cloned sheep" English translation, "cloned strain" English translation, "clonee" English translation, "clonee lakes" English translation, "cloneen" English translation
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